Today I am testing out a glycerol stock I prepared of bacteria containing a plasmid for protein expression. I will be streaking out the bacteria on an agar plate containing antibiotics as selection markers to screen for bacterial colonies only containing plasmid of interest. Plasmids are small circularized DNA which can replicate independently of regular bacterial DNA.
¡Al fin publicada la revisión sistemática! 🎉 Después de hacer la revisión siguiendo una metodología específica, escribir el artículo, cuadrar tablas que a priori parecían imposibles, incontables revisiones del manuscrito y cambios, mandarla a distintas revistas científicas, actualizarla (varias veces), debatir con varios revisores, conseguir que la acepten en una buenísima revista, volver a revisar detalles de formato y esperar meses para verlo publicado... veo un documento de 8 páginas y la sensación es difícil de describir XD
Revisamos los estudios publicados sobre prevalencia de sarcopenia en instituciones residenciales.
Dejo el link por si a alguien le apetece/interesa leerlo:
Y el DOI: 10.1093/advances/nmy058
It's hard to see but this how today is going. Knocked over all my kinetics samples. 40+ tubes all over the floor. Thankfully they were labeled and I had just finished loading my gel.. #researchlife#labrat#postdoclife
What are MACRONUTRIENTS? Part 3!
I’m wrapping up this mini series by talking about FATS! 🥑
Fats, or fatty acids, are found in a variety of forms in our food and are important for cellular structure, transportation, and energy production. Fatty acids all have one end that is hydrophobic (doesn’t like water) and one end that is hydrophilic (it likes water), which is why water and fat doesn’t mix!
Fats can be saturated or unsaturated. Saturated refers to a fat that has no double bonds between carbons, and is therefore “saturated” with hydrogen atoms (like in butter or coconut oil). Unsaturated fats have at least one double bond (monounsaturated fats, like in olive or canola oil) or two or more (polyunsaturated fats, like in walnuts or flax seeds). It is recommended that we limit saturated fats, and increase unsaturated fats for heart health. So yeah, coconut oil isn’t necessarily healthy! 😅🥥
Trans fats, which are usually commercially produced,have recently been banned in the US and other countries. These have bonds that are flipped into a trans position, and have been shown to negatively affect blood lipids. Some foods like meat and dairy contain naturally occurring trans fats, although we aren’t sure if these are necessarily bad for us like those that are commercially produced!
Fats are broken down for energy much more quickly than proteins: your body likes to conserve its liver glycogen to maintain your blood sugar, so it breaks down fats to save its stores! The process of breaking down fats for energy is called beta oxidation, which can produce a lot of energy, but can also produce a lot of reactive oxygen species, which can cause damage to cells. When the body doesn’t have adequate carbohydrates for energy (like when we are fasted), our cells switch to using fatty acids to make adenosine triphosphate, or ATP, which you’ll recall is our cell’s energy “currency.”
There are two fatty acids that our cells cannot make, which means we have to get them from our diet: Omega-3s (alpha-linolenic acid) and Omega-6s (linoleic acid)!
Keep this series in mind for some posts I have coming up 😉 and tag a friend to learn about macronutrients!
TIPS FOR READING A SCIENTIFIC PAPER || I got a big response for a blog post on how to read a scientific article, so here is my most recent post on how to read a scientific paper whether you have a scientific background or not. 📄 📄 📄
I picked a paper that I read recently and found really interesting and made some notes to use as examples of how I go about reading a paper. Check out my newest blog post on getting the most out of reading a research article - the link to the post is in my bio so go check it out and get to reading those papers!
2 6123 hours ago
It's been a week since I splited them but they barely grow... so I screamed at my supervisor and he went down to ask my second supervisor for more cells, that is the moment he found out that we have been using wrong culture medium😂😂😂anyway, I have to throw away all the U2OS cells and restart the colony assy when my second supervisor harvest more fresh cells and the proper medium for me😂😂😂 #sciencefun#cellculture#researchlife#lifesucks
Target vs trial ke-xxx
Never ending & akan terus terjadi selama bekerja sebagai chemist or R&D. Dan... tahun ini mungkin akan jadi lebih berat dari sebelumnya. Dibikin hepi ajalah ya 🤓
And welcome Givaudan buat tooling alatnya, saya siap melayani anda 😂😂
In the work that I do I need to take notes, keep track of meetings, develop new ideas as well as maintain lists of what I’ve found that I still need to read.
#evernote - I use that for maintaining lists of what I should read, ideas about future work, social media posts, articles I want to talk about, as well as taking notes on what I have read. It’s also awesome for capturing contact details from business cards. I use the premium version.
#onenote - I use that for my thinking. Keeping track of thoughts and ideas and how that fit with prevailing thinking as well as earlier ideas. I use it in conjunction with my surface. I find the drawing more enjoying than typing for capturing new ideas. I find it’s much better at the drawing side of things than Evernote. I use it as part of my subscription to Office365
#memomailer - is great for voice recording ideas on the go that I want to send my assistant. They could be things to do. People to meet. Or just how I could improve myself or. How I operate.
#voicerecorder - I used that before I used Evernote and onenote to voice record meetings. I also use #zoom for meetings and that has a great record function. I record meeting so I can get those gems that you somehow miss when note taking. It’s not so much about making sure I have perfect minutes or a perfect record of the meeting. But it could be. Just make sure you get permission to record.